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Field-Induced Alignment
Dr. Chien Ho
Carnegie Mellon University
PDF -
High field allows partial orientation and collection of residual
dipolar coupling data on certain paramagnetic proteins without the aid of
alignment media. |
This proved important in an investigation of
deoxyhemoglobin by Prof. Chien Ho's laboratory. The tetrameric protein is
large (70 kDa) and air sensitive. RDC data allow identification of
alignment axes (see figure) and observation of small deviations from
existing crystal structures. See supplementary figures and the following
publication for details: S. Sahu, V. Simplaceanu, Q. Gong, N. Ho, J.
Glushka, J. Prestegard, and C. Ho, J. Am. Chem. Soc. 128, 6290-6291.
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Resolution and Sensitivity at 900 MHz
Dr. Engin Serpersu University of Tennessee
PDF - Spectra from a 35 kDa protein provide an example of the resolution and sensitivity available at 900 MHz.
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Field-Induced Alignment
Dr. Thomas Weldeghiorghis
Prestegard Lab
University of Georgia
PDF - The field dependence of RDCs is exploited to obtain structural information on a lanthanide tagged protein.
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